1974
- Civitan Club in Gainesville, Fla. was asked to start a community blood bank to support the blood needs of four area hospitals.
1980s
- Suwannee Region opens in Lake City, Fla.
- Citrus Region opens in Lecanto, Fla. and Hernando Region opens in Brooksville, Fla.
- Marion Region opens in Ocala, Fla.
1990s
- Putnam Region opens in Palatka. Fla.
- Birmingham Region opens in Birmingham, Ala.
- Greater Atlanta Region opens in Atlanta, Ga.
- East Alabama Region opens in Opelika, Ala.
- Northeast Georgia Region opens in Gainesville, Ga.
- Montgomery Region opens in Montgomery, Ala.
2000s
- North Alabama Region opens in Huntsville, Ala
- Wiregrass Region opens in Dothan, Ala.
- Marshall Region opens in Albertville, Ala. and Morgan region opens in Decatur, Ala.
- Mobile Region opens in Mobile, Ala.
- Shoals Region opens in Sheffield, Ala., Baldwin Region opens in Daphne, Ala. and Southern Crescent Region in McDonogh, Ga.
2010s
- Valdosta Region opens in Valdosta, Ga.
- First Coast Region opens in Jacksonville, Fla.
2020s
- Tuscaloosa Region opens in Tuscaloosa, Ala.
- The Villages Region opens in The Villages, Fla., Tampa Region opens in Tampa, Fla. and Columbus Region opens in Columbus, Ga.